AKT Ab
Instructions

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AKT Ab
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
AKT Ab
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
AKT Ab
Western blot analysis of AKT1 + AKT2 + AKT3 expression in (1) Hela cell lysate;(2) A549 cell lysate.
AKT Ab
Immunofluorescent analysis using the Antibody at 1:50 dilution.
AKT Ab
Immunofluorescent analysis using the Antibody at 1:50 dilution.
AKT Ab
Immunofluorescent analysis using the Antibody at 1:150 dilution.
AKT Ab
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
AKT Ab
AKT Ab
AKT Ab
AKT Ab
AKT Ab

产品简介

Product Introduction

Catalog No.

CY5425

Source

Recombinant Rabbit mAb

UniProt ID

P31749/P31751/Q9Y243

Reactivity

Human;Mouse;Rat

Application

WB;IHC;ICC/IF;IHF;IP;FC

Calculated Mol Weight

56 kDa

Observed Mol Weight

56 kDa

Recommended Dilution

WB 1:1000-1:2000

IHC 1:100-1:200

ICC/IF 1:50-1:200

IHF 1:50-1:200

IP 1:20-1:50

FC 1:20-1:100

Alternative Names

PKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3; Akt1/2/3; pan Akt;

Host

Rabbit

Isotype

Rabbit IgG

Clonality

Monoclonal

Modification

Unmodified

Immunogen

A synthesized peptide derived from human AKT1

Purification

Affinity-chromatography

Concentration

Buffer System

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Condition

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.

引用文献

Citation

Spatially targeting and regulating tumor-associated macrophages using a raspberry-like micellar system sensitizes pancreatic cancer chemoimmunotherapy

期刊名称：

Nanoscale

PMID：

35972382

影响因子：

8.307

应用范围：

WB

检测物种：

Mouse

发表时间：

2022-07-27

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CY5425

Specifications：

50μl, 150μl

Price：

$188/50μl, $368/150μl

Delivery time：

1-4 weeks

Ordering information：

You can contact authorized dealers to purchase Abways products and obtain technical support, or you can directly contact Abways China

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