p-SMAD3 (S423/S425) Ab

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p-SMAD3 (S423/S425) Ab

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Phospho-Smad3 (S423/S425) expression in A549 cell lysate treated with TGF-ß1.

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunofluorescent analysis of A549 cells treated with TGFβ , using Phospho-Smad3 (S423 + S425) Antibody.

Introduction

Citation

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产品简介

Product Introduction

Catalog No.

CY5140

Source

Recombinant Rabbit mAb

UniProt ID

P84022

Reactivity

Human;Mouse

Application

WB;IHC;ICC/IF;IHF

Calculated Mol Weight

48 kDa

Observed Mol Weight

55 kDa

Recommended Dilution

WB 1:1000-1:2000

IHC 1:100-1:200

ICC/IF 1:50-1:200

IHF 1:50-1:200

Alternative Names

JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

Host

Rabbit

Isotype

Rabbit IgG

Clonality

Monoclonal

Modification

Phosphorylated

Immunogen

A synthesized peptide derived from human SMAD3 around the phosphorylation site of S423/S425

Purification

Affinity-chromatography

Concentration

Buffer System

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Condition

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Description

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.

引用文献

Citation

MiR-20a-3p regulates TGF-β1/Survivin pathway to affect keratinocytes proliferation and apoptosis by targeting SFMBT1 in vitro

期刊名称：

CELLULAR SIGNALLING

PMID：

29886071

影响因子：

3.487

应用范围：

检测物种：

Human

发表时间：

2018-06-07

Tanshinone IIA Activates Nuclear Factor-Erythroid 2-Related Factor 2 to Restrain Pulmonary Fibrosis via Regulation of Redox Homeostasis and Glutaminolysis

期刊名称：

ANTIOXIDANTS & REDOX SIGNALING

PMID：

30105924

影响因子：

5.828

应用范围：

WB,ICC/IF,IHC

检测物种：

Mouse

发表时间：

2019-04-04

Salvia miltiorrhiza Restrains Reactive Oxygen Species-Associated Pulmonary Fibrosis via Targeting Nrf2-Nox4 Redox Balance

期刊名称：

AMERICAN JOURNAL OF CHINESE MEDICINE

PMID：

31352786

影响因子：

3.51

应用范围：

IHC,WB,IP

检测物种：

Mouse

发表时间：

2019-08-15

Ma Xing Shi Gan Decoction Protects against PM2.5-Induced Lung Injury through Suppression of Epithelial-to-Mesenchymal Transition (EMT) and Epithelial Barrier Disruption

期刊名称：

Evidence-based Complementary and Alternative Medicine

PMID：

32655666

影响因子：

1.813

应用范围：

检测物种：

Rat

发表时间：

2020-06-17

miR-744-5p suppresses tumor proliferation and metastasis by targeting transforming growth factor-beta 1 (TGF-β1) in hepatocellular carcinoma (HCC)

期刊名称：

Journal of Gastrointestinal Oncology

PMID：

34532130

影响因子：

2.892

应用范围：

检测物种：

Human

发表时间：

2021-08-01

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CY5140

Specifications：

50μl, 150μl

Price：

$188/50μl, $368/150μl

Delivery time：

1-4 weeks

Ordering information：

You can contact authorized dealers to purchase Abways products and obtain technical support, or you can directly contact Abways China

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