alpha Smooth muscle actin Ab

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alpha Smooth muscle actin Ab

Instructions

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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

Western blot analysis of alpha smooth muscle Actin expression in (1)A549 cell lysate; (2)C2C12 cell lysate using alpha-SMA antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Immunofluorescent analysis using the Antibody at 1:500 dilution.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .

Immunohistofluorescence analysis of Human colon cancer, using the Antibody at 1:100 dilution.

Introduction

Citation

User feedback

产品简介

Product Introduction

Catalog No.

CY1132

Source

Recombinant Rabbit mAb

UniProt ID

P62736

Reactivity

Human;Mouse;Rat

Application

WB;IHC;ICC/IF;IHF;FC

Calculated Mol Weight

42 kDa

Observed Mol Weight

42 kDa

Recommended Dilution

WB 1:1000-1:5000

IHC 1:100-1:200

ICC/IF 1:50-1:200

IHF 1:50-1:200

FC 1:20-1:100

Alternative Names

Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46

Host

Rabbit

Isotype

Rabbit IgG

Clonality

Monoclonal

Modification

Unmodified

Immunogen

A synthesized peptide derived from human alpha Smooth muscle actin

Purification

Affinity-chromatography

Concentration

Buffer System

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Condition

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Description

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

引用文献

Citation

Contribution of acid sphingomyelinase to angiotensin II-induced vascular adventitial remodeling via membrane rafts/Nox2 signal pathway

期刊名称：

LIFE SCIENCES

PMID：

30677425

影响因子：

3.448

应用范围：

IHC,WB,ICC/IF

检测物种：

Rat

发表时间：

2019-01-21

Synergistic cytotoxicity and co-autophagy inhibition in pancreatic tumor cells and cancer-associated fibroblasts by dual functional peptide-modified liposomes

期刊名称：

Acta Biomaterialia

PMID：

31499197

影响因子：

6.638

应用范围：

检测物种：

Mouse,Human

发表时间：

2019-09-06

Mesenchymal stem cells maintain the stemness of colon cancer stem cells via interleukin-8/mitogen-activated protein kinase signaling pathway

期刊名称：

EXPERIMENTAL BIOLOGY AND MEDICINE

PMID：

32122165

影响因子：

3.139

应用范围：

WB,IHC

检测物种：

Human

发表时间：

2020-03-02

Dissecting the single-cell transcriptome network underlying esophagus non-malignant tissues and esophageal squamous cell carcinoma

期刊名称：

EBioMedicine

PMID：

34192657

影响因子：

8.143

应用范围：

IHC

检测物种：

Human

发表时间：

2021-06-27

Glucotoxicity Activation of IL6 and IL11 and Subsequent Induction of Fibrosis May Be Involved in the Pathogenesis of Islet Dysfunction

期刊名称：

Frontiers in Molecular Biosciences

PMID：

34497830

影响因子：

5.246

应用范围：

ICC/IF

检测物种：

Rat

发表时间：

2021-08-23

Co-delivery of autophagy inhibitor and gemcitabine using a pH-activatable core-shell nanobomb inhibits pancreatic cancer progression and metastasis

期刊名称：

Theranostics

PMID：

34522207

影响因子：

11.556

应用范围：

WB,IHC

检测物种：

Mouse,Human

发表时间：

2021-08-04

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CY1132

Specifications：

50μl, 150μl

Price：

$188/50μl, $368/150μl

Delivery time：

1-4 weeks

Ordering information：

You can contact authorized dealers to purchase Abways products and obtain technical support, or you can directly contact Abways China

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