Flag tag Ab - Abways

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Flag tag Ab

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Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate.

Disruption of Ssp411 causes impaired sperm head formation and male sterility in mice. -Biochimica et Biophysica Acta (BBA) - General Subjects

Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution)

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

Introduction

Citation

User feedback

产品简介

Product Introduction

Catalog No.

AB0008

Source

Mouse mAb

UniProt ID

N/A

Reactivity

Recombinant protein

Application

WB;ICC/IF;IP

Calculated Mol Weight

Recombinant protein dependent

Observed Mol Weight

Recombinant protein dependent

Recommended Dilution

WB 1:1000-1:10000

ICC/IF 1:100-1:500

IP 1:50-1:100

Alternative Names

N/A

Host

Mouse

Isotype

Mouse IgG2b

Clonality

Monoclonal

Modification

Unmodified

Immunogen

Recombinant Protein of Flag tag

Purification

Affinity-chromatography

Concentration

Buffer System

Mouse IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Condition

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Description

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

引用文献

Citation

Novel SPEF2 variants cause male infertility and likely primary ciliary dyskinesia

期刊名称：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

PMID：

38568462

影响因子：

3.2

应用范围：

检测物种：

Human

发表时间：

2024-04-03

Membranous translocation of murine double minute 2 promotes the increased renal tubular immunogenicity in ischemia-reperfusion-induced acute kidney injury

期刊名称：

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY

PMID：

38867673

影响因子：

3.7

应用范围：

检测物种：

Human

发表时间：

2024-08-05

Host cells reprogram lipid droplet synthesis through YY1 to resist PRRSV infection

期刊名称：

mBio

PMID：

38953350

影响因子：

5.1

应用范围：

检测物种：

Monkey

发表时间：

2024-07-02

STAT6 promotes innate immunity against BEFV and VSV by inhibiting STUB1 and NIX-mediated MAVS degradation

期刊名称：

VETERINARY MICROBIOLOGY

PMID：

39471658

影响因子：

2.4

应用范围：

WB,COIP

检测物种：

Human,Hamster

发表时间：

2024-10-25

Tufm lactylation regulates neuronal apoptosis by modulating mitophagy in traumatic brain injury

期刊名称：

CELL DEATH AND DIFFERENTIATION

PMID：

39496783

影响因子：

13.7

应用范围：

IP,WB

检测物种：

Human,Mouse

发表时间：

2024-11-05

NEDD4L contributes to ferroptosis and cell growth inhibition in esophageal squamous cell carcinoma by facilitating xCT ubiquitination

期刊名称：

Cell Death Discovery

PMID：

39557844

影响因子：

6.1

应用范围：

WB,CoIP

检测物种：

Human

发表时间：

2024-11-18

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AB0008

Specifications：

50μl, 200μl

Price：

$100/50μl, $288/200μl

Delivery time：

1-4 weeks

Ordering information：

You can contact authorized dealers to purchase Abways products and obtain technical support, or you can directly contact Abways China

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