His tag Ab
Instructions

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His tag Ab
Western blot analysis of His-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with His-tagged protein lysate.
His tag Ab
A PARP1–BRG1–SIRT1 axis promotes HR repair by reducing nucleosome density at DNA damage sites. -NUCLEIC ACIDS RESEARCH
His tag Ab
His tag Ab
His tag Ab
His tag Ab
His tag Ab
His tag Ab

产品简介

Product Introduction

Catalog No.

AB0002

Source

Mouse mAb

UniProt ID

N/A

Reactivity

Recombinant protein

Application

WB;ICC/IF;IP

Calculated Mol Weight

Recombinant protein dependent

Observed Mol Weight

Recombinant protein dependent

Recommended Dilution

WB 1:1000-1:10000

ICC/IF 1:100-1:500

IP 1:50-1:100

Alternative Names

N/A

Host

Mouse

Isotype

Mouse IgG1

Clonality

Monoclonal

Modification

Unmodified

Immunogen

Recombinant Protein of His tag

Purification

Affinity-chromatography

Concentration

Buffer System

Mouse IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Condition

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Description

The His tag antibody is specifically designed for the detection and purification of His-tagged fusion proteins. This antibody recognizes the His tag fused either to the amino- or carboxy- termini of target proteins. It is validated for use in various applications, including Western Blotting (WB), ELISA, and potentially immunoprecipitation and immunocytochemistry.

引用文献

Citation

STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis

期刊名称：

MOLECULAR & CELLULAR PROTEOMICS

PMID：

37146716

影响因子：

7

应用范围：

WB

检测物种：

Human;Mouse

发表时间：

2023-05-03
c-di-GMP inhibits the DNA binding activity of H-NS in Salmonella

期刊名称：

Nature Communications

PMID：

37980414

影响因子：

16.6

应用范围：

WB,IP

检测物种：

发表时间：

2023-11-18
Streptothricin-F Inhibition of FtsZ Function: A Promising Approach for Controlling Pseudomonas syringae pv. actinidiae

期刊名称：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

PMID：

38277222

影响因子：

5.7

应用范围：

WB

检测物种：

Escherichia coli

发表时间：

2024-01-26
Small secreted effector protein from Fusarium sacchari suppresses host immune response by inhibiting ScPi21-induced cell death

期刊名称：

MOLECULAR PLANT PATHOLOGY

PMID：

38279852

影响因子：

4.8

应用范围：

WB

检测物种：

Nicotiana benthamiana

发表时间：

2024-01-11
XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis

期刊名称：

EMBO REPORTS

PMID：

38351372

影响因子：

6.5

应用范围：

ICC/IF,WB

检测物种：

Mouse,Human

发表时间：

2024-02-13

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AB0002

Specifications：

50μl, 200μl

Price：

$100/50μl, $288/200μl

Delivery time：

1-4 weeks

Ordering information：

You can contact authorized dealers to purchase Abways products and obtain technical support, or you can directly contact Abways China

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