APAF 1 Ab
Instructions

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APAF 1 Ab
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
APAF 1 Ab
Western blot analysis of APAF1 expression in (1) HeLa cell lysate; (2) MCF-7 cell lysate.
APAF 1 Ab
Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody .
APAF 1 Ab
Immunofluorescent analysis using the Antibody at 1:50 dilution.
APAF 1 Ab
Immunofluorescent analysis using the Antibody at 1:50 dilution.
APAF 1 Ab
Immunofluorescent analysis using the Antibody at 1:100 dilution.
APAF 1 Ab
APAF 1 Ab

产品简介

Product Introduction

Catalog No.

CY5263

Source

Recombinant Rabbit mAb

UniProt ID

O14727

Reactivity

Human;Mouse

Application

WB;IHC;ICC/IF;IHF

Calculated Mol Weight

142 kDa

Observed Mol Weight

142 kDa

Recommended Dilution

WB 1:1000-1:2000

IHC 1:100-1:200

ICC/IF 1:50-1:200

IHF 1:50-1:200

Alternative Names

APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413

Host

Rabbit

Isotype

Rabbit IgG

Clonality

Monoclonal

Modification

Unmodified

Immunogen

A synthesized peptide derived from human APAF 1

Purification

Affinity-chromatography

Concentration

Buffer System

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Condition

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Description

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

引用文献

Citation

Circadian gene Clock participates in mitochondrial apoptosis pathways by regulating mitochondrial membrane potential, mitochondria out membrane permeablization and apoptosis factors in AML12 hepatocytes

期刊名称：

MOLECULAR AND CELLULAR BIOCHEMISTRY

PMID：

32067140

影响因子：

2.795

应用范围：

WB

检测物种：

Mouse

发表时间：

2020-02-17

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CY5263

Specifications：

50μl, 150μl

Price：

$188/50μl, $368/150μl

Delivery time：

1-4 weeks

Ordering information：

You can contact authorized dealers to purchase Abways products and obtain technical support, or you can directly contact Abways China

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