Recombinant BMP-14 (Bone morphogenetic protein-14), Human ,AF
Source: Cytokines Catalog No. : PY2112
Recombinant BMP-14 (Bone morphogenetic protein-14), Human ,AF (PY2112)
UniProt:P43026
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Source:Human Cytokineshttp://www.abways.com/showproduct.asp?cid=PY2112
Data Sheet
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UniProt
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Gene ID8200
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Calculated Molecular Weight14.52 kDa
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SDS-PAGE Molecular Weight(under reducing condition)18 kDa
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Alternative NamesCartilage-Derived Morphogenetic Protein-1,Growth/Differentiation Factor-5,GDF-5, CDMP-1
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ActivityMeasure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <14 ng/mL.
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionBone morphogenetic proteins (BMPs) are a group of growth factors also known as cytokines and as metabologens. BMP-14 is a principal inhibitor of cartilage development and is predominantly expressed in long bone during human embryonic development. Recombinant human BMP-14 is a 27 kDa homodimeric protein consisting of two 120 amino acid polypeptide chains.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR with polyhistidine tag at the C-terminus.