Lamin A/C Antibody
Source: Monoclonal Antibodies Catalog No. : CY5222
Lamin A/C Antibody (CY5222)
UniProt:P02545
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.com/showproduct.asp?cid=CY5222
Data Sheet
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UniProt
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Mol Weight63kDa/74kDa
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Alternative Names70 kDa lamin;LAMA,;LMN1;LMN C; lamin A/C; Lamin; HGPS; EMD2;
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ImmunogenA synthesized peptide derived from human Lamin A/C
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Play an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.
Applications
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ReactivityHuman
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:500 dilution. -
Western blot analysis of Lamin A/C expression in HeLa whole cell lysates. -
Immunohistochemical analysis of paraffin-embedded human kidney, using Lamin A/C Antibody.
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