Recombinant VEGF (Vascular endothelial growth factor), Swine ,AF
Source: Cytokines Catalog No. : PY2924
Recombinant VEGF (Vascular endothelial growth factor), Swine ,AF (PY2924)
UniProt:P49151
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Source:Swine Cytokineshttp://www.abways.com/showproduct.asp?cid=PY2924
Data Sheet
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UniProt
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Gene ID397157
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Calculated Molecular Weight20.16 kDa
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SDS-PAGE Molecular Weight(under reducing condition)17-25 kDa
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Alternative NamesVEGFA
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ActivityTesting in process
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>95% as determined by SDS-PAGE. Ni-NTA chromatography.
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionVascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMAPMAEGDQKPHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEEFNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR with polyhistidine tag at the C-terminus.