Recombinant TGF beta 1 (Transforming growth factor beta 1), Swine ,AF
Source: Cytokines Catalog No. : PY2922
Recombinant TGF beta 1 (Transforming growth factor beta 1), Swine ,AF (PY2922)
UniProt:P07200
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Source:Swine Cytokineshttp://www.abways.com/showproduct.asp?cid=PY2922
Data Sheet
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UniProt
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Gene ID397078
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Calculated Molecular Weight13.7 kDa
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SDS-PAGE Molecular Weight(under reducing condition)11-17 kDa
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Alternative NamesTGF-BETA-1, TGFB1
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ActivityMeasure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED50 for this effect is <0.1 ng/mL.
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionTransforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HC
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus.