Recombinant CXCL5 (C-X-C motif chemokine 5), Human ,AF
Source: Cytokines Catalog No. : PY2143
Recombinant CXCL5 (C-X-C motif chemokine 5), Human ,AF (PY2143)
UniProt:P42830
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Source:Human Cytokineshttp://www.abways.com/showproduct.asp?cid=PY2143
Data Sheet
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UniProt
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Gene ID6374
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Calculated Molecular Weight8.51 kDa
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SDS-PAGE Molecular Weight(under reducing condition)12 kDa
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Alternative NamesEpithelial Neutrophil Activating Peptide-78, ENA-78
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ActivityMeasure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED50 for this effect is <10 ng/mL.
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the N-terminus
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Expression HostEscherichia coli
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DescriptionThe protein encoded by this gene, Chemokine (C-X-C motif) ligand 5 (CXCL5), is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). It is observed that, CXCL5 also expresses in eosinophils, and can interact with the type II interferon IFN-?, thereby cause an inhibition. This chemokine stimulates the chemotaxis of neutrophils possesses angiogenic properties, and elicits these effects by interacting with the cell surface chemokine receptor CXCR2.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceRELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN with polyhistidine tag at the N-terminus.