Recombinant Annexin V, Human ,AF
Source: Cytokines Catalog No. : PY2104
Recombinant Annexin V, Human ,AF (PY2104)
UniProt:P08758
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Source:Human Cytokineshttp://www.abways.com/showproduct.asp?cid=PY2104
Data Sheet
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UniProt
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Gene ID308
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Calculated Molecular Weight36.75 kDa
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SDS-PAGE Molecular Weight(under reducing condition)37 kDa
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Alternative NamesANXA5, ANX5, ENX2, HEL-S-7, PP4, RPRGL3
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ActivityTesting in process
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionAnnexin V is a cellular protein in the annexin group. In flow experiment, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane. Annexin V has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1.
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Formulationdown/PY2104.pdf
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ReconstitutionThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETL
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SDS- PAGE analysis of recombinant human Annexin V
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