Recombinant AITRL (Activation-induced TNFR member ligand), Human ,AF
Source: Cytokines Catalog No. : PY2103
Recombinant AITRL (Activation-induced TNFR member ligand), Human ,AF (PY2103)
UniProt:Q9UNG2
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Source:Human Cytokineshttp://www.abways.com/showproduct.asp?cid=PY2103
Data Sheet
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UniProt
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Gene ID8995
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Calculated Molecular Weight15.34 kDa
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SDS-PAGE Molecular Weight(under reducing condition)11 kDa
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Alternative NamesTL6, GITRL, TNLG2A, hGITRL, TNFSF18
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ActivityMeasure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <2.5 ng/mL.
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionAITRL is a member of the TNF superfamily which is expressed in endothelial cells and signals through the AITR receptor. AITRL regulates T-cell proliferation and survival. It also effectuates the interaction between T lymphocytes and endothelial cells. The AITRL gene codes type II transmembrane protein comprised of 177 amino acids, including a 28 amino acid cytoplasmic region, a 21 amino acid transmembrane domain and a 128 amino acid extracellular domain.
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Formulationdown/PY2103.pdf
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ReconstitutionThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMQLETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGII LIANPQEI with polyhistidine tag at the C-terminus.