Oct4 Antibody
Source: Monoclonal Antibodies Catalog No. : CY5781
Oct4 Antibody (CY5781)
UniProt:Q01860
Application:WB,IHC,ICC/IF,IP,ChIP
Reactivity:Human,Mouse
Source:Rabbit mAb
References（2）
http://www.abways.com/showproduct.asp?cid=CY5781
Data Sheet
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UniProt
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Mol Weight45kDa
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Alternative NamesOctamer binding protein 3; Octamer binding protein 4; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; POU5F1;
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ImmunogenA synthesized peptide derived from human Oct4
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionOct-4 is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Applications
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ReactivityHuman,Mouse
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ApplicationWB,IHC,ICC/IF,IP,ChIP
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Recommended dilutionWB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 ChIP
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All lanes use the Antibody at 1:2k dilution for 1 hour at room temperature. -
Western blot analysis of Oct4 expression in NCCIT cell lysate. -
Immunohistochemical analysis of paraffin-embedded human seminoma, using Oct4 Antibody. -
Immunofluorescent analysis of NCCIT cells, using Oct4 Antibody.
References （2）
ONECUT2 which is targeted by hsa-miR-15a-5p enhances stemness maintenance of gastric cancer stem cells-Experimental Biology and Medicine (EXP BIOL MED)
IL-8/CXCR2 mediates tropism of human bone marrow-derived mesenchymal stem cells toward CD133+/CD44+ Colon cancer stem cells-Journal of Cellular Physiology (J Cell Physiol)
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