β-Actin Mouse Monoclonal Antibody_Polyclonal Antibodies-Abways Technology

β-Actin Mouse Monoclonal Antibody

Source: Polyclonal Antibodies Catalog No. : AY0573

β-Actin Mouse Monoclonal Antibody (AY0573)
UniProt:P60709
Application:WB,IHC,IF
Reactivity:Human,Monkey,Mouse,Rat,hamster,Dog,Spodoptera frugiperda,chicken,rabbit
Source:Mouse mAb
References（6）
http://www.abways.com/showproduct.asp?cid=AY0573

 Datasheet

Data Sheet

UniProt

P60709
Mol Weight

43kDa
Alternative Names

ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Immunogen

A synthesized peptide derived from human β-Actin
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Applications

Reactivity

Human,Monkey,Mouse,Rat,hamster,Dog,Spodoptera frugiperda,chicken,rabbit
Application

WB,IHC,IF
Recommended dilution

WB: 1:3000-10000 IHC: 1:200 IF: 1:200

Western blot analysis of extracts from Hela cell lysate(lane1) , Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3), using β-Actin Mouse Monoclonal antibody.
Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using β-Actin Mouse Monoclonal antibody.

References （6）

Vinpocetine Attenuates Ischemic Stroke Through Inhibiting NLRP3 Inflammasome Expression in Mice-JOURNAL OF CARDIOVASCULAR PHARMACOLOGY (J Cardiovasc Pharmacol)

Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels-Scientific Reports (SCI REP-UK)

Tissue-specific Nutrient Metabolism in the Liver and Muscle of Juvenile Blunt Snout Bream (Megalobrama Amblycephala) in Response to Dietary Methionine Levels-scientific reports

Interactive effects of water salinity and dietary methionine levels on growth performance, whole-body composition, plasma parameters, and expression of major nutrient metabolism genes in juvenile genetically improved farmed Tilapia (Oreochromis niloticus)

Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro-Chinese Medicine (CHIN MED-UK)

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