HP1 gamma/CBX3 (6D8) Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3614
HP1 gamma/CBX3 (6D8) Mouse mAb (AB3614)
UniProt:Q13185
Application:WB,IHC
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3614
Data Sheet
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UniProt
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Mol Weight24kDa
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Alternative NamesCBX3; Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein
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ImmunogenPurified recombinant protein expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.Q13185.Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitement of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC
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Recommended dilutionWB: 1/500-1/1000 IHC: 1/50-1/100
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Western blot analysis of HP1 gamma (6D8) in MCF7,Raw264.7,Hela lysates using HP1 gamma (6D8) antibody.
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Immunohistochemistry analysis of paraffinembedded Human Colon Carcinoma Tissue using HP1 gamma (6D8) antibody.
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Immunohistochemical analysis of paraffinembedded human tonsils using HP1 gamma (6D8) antibody.