BRG1 Mouse mAb_Monoclonal Antibodies-Abways Technology

Size: 50μl, 100μl
Price: $185/50μl, $299/100μl
Delivery: 3-4 days

BRG1 Mouse mAb

Source: Monoclonal Antibodies Catalog No. : AB3305

BRG1 Mouse mAb (AB3305)
UniProt:P51532
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3305

 Datasheet

Data Sheet

UniProt

P51532
Mol Weight

220KDa
Alternative Names

ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta;
Immunogen

Purified recombinant human BRG1 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation.Component of the CREST-BRG1 complex,a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex.

Applications

Reactivity

Human,Mouse
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:1000 ICC/IF 1:50 IP

Western blot detection of BRG1 in Hela NE,F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-BRG1 mouse mAb (dilution 1:50). DAPI was used to stain nucleus(blue).
Immunoprecipitation analysis of K562 cell lysates using BRG1 mouse mAb (201025).