BiP/GRP78(C-term) Mouse mAb_Monoclonal Antibodies-Abways Technology

Size: 50μl, 100μl
Price: $185/50μl, $299/100μl
Delivery: 3-4 days

BiP/GRP78(C-term) Mouse mAb

Source: Monoclonal Antibodies Catalog No. : AB3142

BiP/GRP78(C-term) Mouse mAb (AB3142)
UniProt:P11021
Application:WB,IHC,ICC/IF
Reactivity:Human,Rat
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3142

 Datasheet

Data Sheet

UniProt

P11021
Mol Weight

78KDa
Alternative Names

GRP-78; GRP78; BIP; MIF2; FLJ26106; HSPA5
Immunogen

Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER

Applications

Reactivity

Human,Rat
Application

WB,IHC,ICC/IF
Recommended dilution

WB 1:1000 IHC 1:100 ICC/IF 1:50

Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.
Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).