EpCAM Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3139
EpCAM Mouse mAb (AB3139)
UniProt:P16422
Application:WB,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3139
Data Sheet
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UniProt
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Mol Weight39KDa
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Alternative NamesESA; KSA; M4S1; MK1; DIAR5; EGP2;EGP40; KS1/4; MIC18; TROP1; TACSTD1; EPCAM;
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ImmunogenPurified recombinant EpCAM protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionMay act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Applications
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ReactivityHuman
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ApplicationWB,IP
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Recommended dilutionWB 1:1000 IP
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Western blot analysis of extracts from CHO-K1,CHO-K1 transfected by Flag-EpCAM, MCF7(EpCAM positive),HCT116(EpCAM positive),and Hela(EpCAM negative) cell lysates using EpCAM mouse mAb (1:1000 diluted).Predicted band size:39KDa.Observed band size:39/35KDa.
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Immunoprecipitation analysis of HCT116 and Hela cell lysates using EpCAM.