pyruvate dehydrogenase (lipoamide) α 1 Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3131
pyruvate dehydrogenase (lipoamide) α 1 Mouse mAb (AB3131)
UniProt:P08559
Application:WB,ICC/IF
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3131
Data Sheet
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UniProt
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Mol Weight43KDa
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Alternative NamesPyruvate dehydrogenase E1 component subunit alpha, somatic form; mitochondrial; PDHE1-A type I; PDHA1; PHE1A;
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ImmunogenPurified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Applications
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ReactivityHuman,Mouse
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:1000 ICC/IF 1:100
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Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293,1299,mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa. -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb(dilution 1:100).
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