SMC1A(C-term) Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3129
SMC1A(C-term) Mouse mAb (AB3129)
UniProt:Q14683
Application:WB,ICC/IF
Reactivity:Human
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3129
Data Sheet
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UniProt
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Mol Weight143KDa
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Alternative NamesChromosome segregation protein SmcB; DXS423E; KIAA0178; SB1.8/DXS423E protein; SM1A; SMC1A; SMC1L1; SMC1alpha protein;
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ImmunogenPurified recombinant human SMC1A(C-term.) protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionInvolved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:1000 ICC/IF 1:100
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Western blot detection of SMC1A(C-term) in MOLT-4,U87 MG and HelaNE cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa. -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-SMC1A mouse mAb(dilution 1:100).
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