Sox2 Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3118
Sox2 Mouse mAb (AB3118)
UniProt:P48431
Application:WB,ICC/IF,FC
Reactivity:Mouse
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3118
Data Sheet
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UniProt
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Mol Weight35KDa
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Alternative NamesSOX2; ANOP3; MCOPS3; MGC2413; lcc; SRY related HMG box 2;
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ImmunogenPurified recombinant mouse Sox2 protein fragments expressed in E.coli
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206 by similarity.Critical for early embryogenesis and for embryonic stem cell pluripotency.May function as a switch in neuronal development.
Applications
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ReactivityMouse
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ApplicationWB,ICC/IF,FC
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Recommended dilutionWB 1:1000 ICC/IF 1:150 FC 1:100
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Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.
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Immunofluorescent analysis of COS7 cells using anti-Sox2 mouse mAb diluted 1:150.
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Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.