POU5F1/OCT4 Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3117
POU5F1/OCT4 Mouse mAb (AB3117)
UniProt:Q01860
Application:WB
Reactivity:Mouse
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3117
Data Sheet
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UniProt
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Mol Weight45KDa
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Alternative NamesOctamer binding protein 3; Octamer binding protein 4; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4;
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ImmunogenPurified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3').Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206.Critical for early embryogenesis and for embryonic stem cell pluripotency.
Applications
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ReactivityMouse
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ApplicationWB
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Recommended dilutionWB 1:1000
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Western blot detection of POU5F1/OCT4 in F9 cell lysates using POU5F1/OCT4 mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.
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