HP1-α Mouse mAb
Source: Monoclonal Antibodies  Catalog No. : AB3114 
HP1-α Mouse mAb (AB3114)
UniProt:P45973
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3114
Data Sheet
  • UniProt
  • Mol Weight
    26KDa
  • Alternative Names
    CBX5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha;
  • Immunogen
    Purified recombinant human HP1 alpha protein fragments expressed in E.coli.
  • Storage
    Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purification
    Affinity-chromatography
  • Isotype
    Mouse IgG2b
  • Clonality
    Monoclonal
  • Description
    Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR).
Applications
  • Reactivity
    Human,Mouse
  • Application
    WB,IHC,ICC/IF
  • Recommended dilution
    WB 1:1000 IHC 1:200 ICC/IF 1:300
  • Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).
  • Immunofluorescent analysis stain of Hela using HP1 alpha mouse mAb (1:300).
Catalog No:AB3114
  • Size:50μl, 100μl
  • Price:$185/50μl, $299/100μl
  • Delivery:3-4 days
Order Information:
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