HP1-α Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3114
HP1-α Mouse mAb (AB3114)
UniProt:P45973
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3114
Data Sheet
-
UniProt
-
Mol Weight26KDa
-
Alternative NamesCBX5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha;
-
ImmunogenPurified recombinant human HP1 alpha protein fragments expressed in E.coli.
-
StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
PurificationAffinity-chromatography
-
IsotypeMouse IgG2b
-
ClonalityMonoclonal
-
DescriptionComponent of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR).
Applications
-
ReactivityHuman,Mouse
-
ApplicationWB,IHC,ICC/IF
-
Recommended dilutionWB 1:1000 IHC 1:200 ICC/IF 1:300
-
Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa
-
Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).
-
Immunofluorescent analysis stain of Hela using HP1 alpha mouse mAb (1:300).