HP1-γ Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3113
HP1-γ Mouse mAb (AB3113)
UniProt:Q13185
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Monkey,Hamster,Rat
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3113
Data Sheet
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UniProt
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Mol Weight22KDa
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Alternative NamesCBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; HP1 gamma; HP1Hs gamma; Modifier 2 protein;
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ImmunogenPurified recombinant human HP1-gamma protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR).
Applications
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ReactivityHuman,Mouse,Monkey,Hamster,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:1000 IHC 1:200 ICC/IF 1:200 IP
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Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa. -
Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0). -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200). -
Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.
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