Eg5 Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3108
Eg5 Mouse mAb (AB3108)
UniProt:P52732
Application:WB,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3108
Data Sheet
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UniProt
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Mol Weight130KDa
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Alternative NamesEG5; HKSP; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; KNSL1; MCLMR; TR-interacting protein 5; TRIP5;
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ImmunogenPurified recombinant human Eg5 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionMotor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:1000 ICC/IF 1:200 IP
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Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa. -
Immunofluorescent analysis of Hela using Eg5 mouse mAb (1:200). -
Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.
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