KDM1/LSD1 Mouse mAb
Source: Monoclonal Antibodies Catalog No. : AB3103
KDM1/LSD1 Mouse mAb (AB3103)
UniProt:O60341
Application:WB,ICC/IF,IP
Reactivity:Human,Monkey
Source:Mouse mAbhttp://www.abways.com/showproduct.asp?cid=AB3103
Data Sheet
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UniProt
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Mol Weight110KDa
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Alternative NamesKDM1;AOF2;BHC110;KIAA0601;LSD1; KDM1A;
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ImmunogenPurified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3,thereby acting as a coactivator or a corepressor,depending on the context.Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.Acts as a corepressor by mediating demethylation of H3K4me,a specific tag for epigenetic transcriptional activation.
Applications
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ReactivityHuman,Monkey
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:1000 ICC/IF 1:100 IP
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Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa. -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-KDM1/LSD1 mouse mAb (dilution 1:100). -
Immunoprecipitation analysis of Hela cell lysates using KDM1/LSD1 mouse mAb.
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