beta Actin Antibody(HRP Conjugated)_Tags and Loading Controls-Abways Technology

beta Actin Antibody(HRP Conjugated)

Source: Tags and Loading Controls Catalog No. : AB2001

beta Actin Antibody(HRP Conjugated) (AB2001)
UniProt:P60709
Application:WB
Reactivity:Human,Mouse,Monkey,Rat,Hamster,Plant
Source:Mouse mAb
References（11）
http://www.abways.com/showproduct.asp?cid=AB2001

 Datasheet

Data Sheet

UniProt

P60709
Mol Weight

42kDa
Alternative Names

ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;
Immunogen

A synthesized peptide derived from human beta Actin
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG2b
Clonality

Monoclonal
Description

beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Applications

Reactivity

Human,Mouse,Monkey,Rat,Hamster,Plant
Application

WB
Recommended dilution

WB 1:3000~1:10000

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Western blot analysis of beta Actin expression in (1) Hela cell lysate, (2) Human liver lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) PC12 cell lysate, (6) Rat heart lysate.
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.

References （11）

Saturated fatty acids activate the inflammatory signalling pathway in Schwann cells: Implication in sciatic nerve injury-SCANDINAVIAN JOURNAL OF IMMUNOLOGY (SCAND J IMMUNOL)

An electrochemical biosensor for the detection of epithelial-mesenchymal transition-Nature Communications (NAT COMMUN)

Design, synthesis and discovery of 2(1H)-quinolone derivatives for the treatment of pulmonary fibrosis through inhibition of TGF-β/smad dependent and independent pathway-European Journal of Medicinal Chemistry (EUR J MED CHEM)

House Dust Mite Induces IL-33 Release From Human Nasal Epithelial Cells via P2Y Purinergic Signals and ERK/P38 MAPK Pathways.-research square

Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury-Theranostics

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