GAPDH Antibody(HRP conjugated)
Source: Tags and Loading Controls Catalog No. : AB2000
GAPDH Antibody(HRP conjugated) (AB2000)
UniProt:P04406
Application:WB
Reactivity:Human,Mouse,Rat,Mk,Ch,Ze,Fish
Source:Rabbit mAb
References（15）
http://www.abways.com/showproduct.asp?cid=AB2000
Data Sheet
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UniProt
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Mol Weight36kDa
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Alternative Namesaging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685
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ImmunogenA synthesized peptide derived from human GAPDH(HRP conjugated)
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting.
Applications
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ReactivityHuman,Mouse,Rat,Mk,Ch,Ze,Fish
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ApplicationWB
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Recommended dilutionWB 1:5000~1:20000
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat -
Constitutive Activation of β-Catenin in Differentiated Osteoclasts Induces Bone Loss in Mice. -Cell Physiol Biochem
References （15）
Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.-Oncology Reports
Constitutive Activation of β-Catenin in Differentiated Osteoclasts Induces Bone Loss in Mice.-Cell Physiol Biochem
Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury-Stem Cell Research & Therapy(STEM CELL RES THER)
Dopamine D1 receptor agonist A68930 attenuates acute kidney injury by inhibiting NLRP3 inflammasome activation-Journal of Pharmacological Sciences (J PHARMACOL SCI)
Tempo-spatial alternative polyadenylation analysis reveals that 3′ UTR lengthening of Mdm2 regulates p53 expression and cellular senescence in aged rat testis-Biochemical and Biophysical Research Communications (BIOCHEM BIOPH RES CO)
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