Goat Anti-Mouse IgG (H+L) FITC
Source: Secondary Antibodies Catalog No. : AB0122
Goat Anti-Mouse IgG (H+L) FITC (AB0122)
UniProt:N/A
Application:IHC ICC/IF FC
Reactivity:Mouse
Source:Goat IgG
References（3）
http://www.abways.com/showproduct.asp?cid=AB0122
Data Sheet
-
UniProt
-
Mol WeightN/A
-
Alternative NamesFluorescein (FITC)；FITC
-
ImmunogenGoat IgG
-
StorageGoat IgG in 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
PurificationAffinity-chromatography
-
IsotypeGoat IgG
-
ClonalityPolyclonal
-
DescriptionFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor 488 because it is brighter and more photostable.
Applications
-
ReactivityMouse
-
ApplicationIHC ICC/IF FC
-
Recommended dilutionIHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:50-1:200
-
Immunofluorescent analysis of Hela cells.
References （3）
Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats-Acta Pharmacologica Sinica (ACTA PHARMACOL SIN)
Characterization of a novel cysteine protease in Trichinella spiralis and its role in larval intrusion, development and fecundity-Veterinary Research (VET RES)
Molecular characterization of a novel cathepsin L from Trichinella spiralis and its participation in invasion, development and reproduction-Acta Tropica (ACTA TROP)
Communicate
One click phone