beta Actin Mouse Monoclonal Antibody
Source: Tags and Loading Controls Catalog No. : AB0061
beta Actin Mouse Monoclonal Antibody (AB0061)
UniProt:P60709
Application:WB
Reactivity:Human,Mouse,Monkey,Goat,Rat,Hamster,Saccharomyces,cerevisiae,Arabidopsis,Pichia,pastoris,Rabbit,Zebrafish,Drosophila,Leaf,of,Rice,Fruit,of,Cucumber,Chlamydomonas,Reinhardtii
Source:Mouse mAb
References(3)
http://www.abways.com/showproduct.asp?cid=AB0061
Data Sheet
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UniProt
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Mol Weight42kDa
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Alternative NamesACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;
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ImmunogenA synthesized peptide derived from human beta Actin
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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Descriptionbeta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Applications
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ReactivityHuman,Mouse,Monkey,Goat,Rat,Hamster,Saccharomyces,cerevisiae,Arabidopsis,Pichia,pastoris,Rabbit,Zebrafish,Drosophila,Leaf,of,Rice,Fruit,of,Cucumber,Chlamydomonas,Reinhardtii
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ApplicationWB
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Recommended dilutionWB 1:3000~1:10000
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Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.
References (3)
Induction of the Unfolded Protein Response during Bovine Alphaherpesvirus 1 Infection-viruses (Viruses-Basel VIRUSES-BASEL)
Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid-British Journal of Nutrition (BRIT J NUTR)
Metformin inhibits EV71‑induced pyroptosis by upregulating DEP domain‑containing mTOR‑interacting protein-Experimental and Therapeutic Medicine