beta Actin Mouse Monoclonal Antibody
Source: Tags and Loading Controls  Catalog No. : AB0061 
beta Actin Mouse Monoclonal Antibody (AB0061)
UniProt:P60709
Application:WB
Reactivity:Human,Mouse,Monkey,Goat,Rat,Hamster,Saccharomyces,cerevisiae,Arabidopsis,Pichia,pastoris,Rabbit,Zebrafish,Drosophila,Leaf,of,Rice,Fruit,of,Cucumber,Chlamydomonas,Reinhardtii
Source:Mouse mAb
References(3)
http://www.abways.com/showproduct.asp?cid=AB0061
Data Sheet
  • UniProt
  • Mol Weight
    42kDa
  • Alternative Names
    ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;
  • Immunogen
    A synthesized peptide derived from human beta Actin
  • Storage
    Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purification
    Affinity-chromatography
  • Isotype
    Mouse IgG2b
  • Clonality
    Monoclonal
  • Description
    beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Applications
  • Reactivity
    Human,Mouse,Monkey,Goat,Rat,Hamster,Saccharomyces,cerevisiae,Arabidopsis,Pichia,pastoris,Rabbit,Zebrafish,Drosophila,Leaf,of,Rice,Fruit,of,Cucumber,Chlamydomonas,Reinhardtii
  • Application
    WB
  • Recommended dilution
    WB 1:3000~1:10000
  • Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.
References 3
Induction of the Unfolded Protein Response during Bovine Alphaherpesvirus 1 Infection-viruses (Viruses-Basel VIRUSES-BASEL)
Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid-British Journal of Nutrition (BRIT J NUTR)
Metformin inhibits EV71‑induced pyroptosis by upregulating DEP domain‑containing mTOR‑interacting protein-Experimental and Therapeutic Medicine
Catalog No:AB0061
  • Size:50μl, 100μl
  • Price:$180/100μl
  • Delivery:Ship next day
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