beta Tubulin Antibody_Tags and Loading Controls-Abways Technology

beta Tubulin Antibody

Source: Tags and Loading Controls Catalog No. : AB0057

beta Tubulin Antibody (AB0057)
UniProt:P07437
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat,Cow,Dog,Chicken,Monkey,Zebrafish
Source:Rabbit mAb
References（1）
http://www.abways.com/showproduct.asp?cid=AB0057

 Datasheet

Data Sheet

UniProt

P07437
Mol Weight

50kDa
Alternative Names

Beta 4 tubulin; Beta 5 tubulin; Beta1 tubulin; Tubulin beta chain; Tubulin beta class I;
Immunogen

A synthesized peptide derived from human beta Tubulin
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Applications

Reactivity

Human,Mouse,Rat,Cow,Dog,Chicken,Monkey,Zebrafish
Application

WB,IHC,ICC/IF,FC
Recommended dilution

WB 1:3000~1:10000 IHC 1:100~1:200 ICC/IF 1:100~1:500 FC 1:50

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:250 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of beta Tubulin expression in (1) Hela cell lysate; (2) RAW 264.7 lysate.

References （1）

The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication-Autophagy