GAPDH Antibody
Source: Tags and Loading Controls  Catalog No. : AB0037  
GAPDH Antibody  (AB0037)
UniProt:P04406
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat,Mk,Ch,Ze,Fish
Source:Rabbit mAb
References（124）
http://www.abways.com/showproduct.asp?cid=AB0037
Data Sheet
  • UniProt
    P04406
  • Mol Weight
    36kDa
  • Alternative Names
    aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685;
  • Immunogen
    A synthesized peptide derived from human GAPDH
  • Storage
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purification
    Affinity-chromatography
  • Isotype
    Rabbit IgG
  • Clonality
    Monoclonal
  • Description
    Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Applications
  • Reactivity
    Human,Mouse,Rat,Mk,Ch,Ze,Fish
  • Application
    WB,IHC,ICC/IF,IP,FC
  • Recommended dilution
    WB 1:5000~1:50000 IHC 1:100~1:500 ICC/IF 1:100~1:250 IP 1:50 FC 1:50
  • AB0037
    Immunohistochemical analysis of paraffin-embedded Human pituitary adenoma, using the Antibody at 1:400 dilution.
  • AB0037
    Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:400 dilution.
  • AB0037
    Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:400 dilution.
  • AB0037
    Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Antibody.
  • AB0037
    All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.
  • AB0037
    All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.
  • AB0037
    LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway. -Journal of Experimental & Clinical Cancer Research
  • AB0037
    Arsenic circumvents the gefitinib resistance by binding to P62 and mediating autophagic degradation of EGFR in non-small cell lung cancer. -Cell Death and Disease
  • AB0037
    Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry
References （124）
Effects of Cold Stress on the Photosynthesis and Antioxidant System of Rhododendron chrysanthum Pall.-biology and life sciences
The transcription factor GATA3 is required for homologous recombination repair by regulating CtIP expression.-Oncogene
FoxM1 overexpression promotes cell proliferation and migration and inhibits apoptosis in hypopharyngeal squamous cell carcinoma resulting in poor clinical prognosis.-INTERNATIONAL JOURNAL OF ONCOLOGY
LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway.-Journal of Experimental & Clinical Cancer Research
A Disintegrin and Metalloproteinase with Thrombospondin Motifs 18 Deficiency Leads to Visceral Adiposity and Associated Metabolic Syndrome in Mice.-The American Journal of Pathology
Catalog No:AB0037
  • Size:50μl, 100μl
  • Price:$180/100μl
  • Delivery:Ship next day
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