GAPDH Antibody
Source: Tags and Loading Controls Catalog No. : AB0036
GAPDH Antibody (AB0036)
UniProt:P04406
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat,Chicken,Cow,Dog,Monkey
Source:Rabbit mAb
References（27）
http://www.abways.com/showproduct.asp?cid=AB0036
Data Sheet
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UniProt
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Mol Weight36kDa
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Alternative Namesaging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685
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ImmunogenA synthesized peptide derived from human GAPDH
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Applications
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ReactivityHuman,Mouse,Rat,Chicken,Cow,Dog,Monkey
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:3000~1:10000 IHC 1:100~1:250 ICC/IF 1:100~1:250
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Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody. -
Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody. -
Immunofluorescent analysis of Hela cells, using GAPDH Antibody.
References （27）
Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.-Oncology Reports
Inhibited autophagy impairs systemic nutrient metabolism in Nile tilapia.-Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Transcriptional Regulation of Selenoprotein F by Heat Shock Factor 1 during Selenium Supplementation and Stress Response.-Cells
Self-Assembled/Drug-Composed Nanomedicine for Synergistic Photonic Hyperthermia and Targeted Therapy of Breast Cancer by Inhibiting ERK, AKT, and STAT3 Signaling Cascades-VETERINARY RESEARCH(VET RES)
The regulation of rapamycin on nutrient metabolism in Nile tilapia fed with high-energy diet-Veterinary Microbiology (VET MICROBIOL)
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