beta Actin Mouse Monoclonal Antibody
Source: Tags and Loading Controls Catalog No. : AB0011
beta Actin Mouse Monoclonal Antibody (AB0011)
UniProt:P60709
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat,Mk,Dg,Ch,Pg,Rabbit
Source:Mouse mAb
References（10）
http://www.abways.com/showproduct.asp?cid=AB0011
Data Sheet
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UniProt
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Mol Weight42kDa
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Alternative NamesN/A
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ImmunogenA synthesized peptide derived from human beta Actin
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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Descriptionbeta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Applications
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ReactivityHuman,Mouse,Rat,Mk,Dg,Ch,Pg,Rabbit
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:3000~1:10000 IHC 1:100~1:200 ICC/IF 1:100~1:200
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Western blot analysis of beta Actin expression in (1) Hela cell lysate; (2) Rat brain lysate; (3) Mouse brain lysate; (4) Chicken lung lysate; (5) Rabbit testis lysate with beta Actin mouse mAb -
Immunohistochemical analysis of paraffin-embedded human heart, using beta Actin Mouse Monoclonal Antibody. -
Immunofluorescent analysis of HepG2 cells, using beta Actin mAb. -
Analysis of miRNA–mRNA network reveals miR-140-5p as a suppressor of breast cancer glycolysis via targeting GLUT1. -EPIGENOMICS -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
References （10）
Analysis of miRNA–mRNA network reveals miR-140-5p as a suppressor of breast cancer glycolysis via targeting GLUT1.-EPIGENOMICS
Macrophage MSR1 promotes BMSC osteogenic differentiation and M2-like polarization by activating PI3K/AKT/GSK3β/β-catenin pathway-Theranostics
Macrophage MSR1 promotes the formation of foamy macrophage and neuronal apoptosis after spinal cord injury-Journal of Neuroinflammation (J NEUROINFLAMM )
Macrophage GIT1 Contributes to Bone Regeneration by Regulating Inflammatory Responses in an ERK/NRF2‐Dependent Way-JOURNAL OF BONE AND MINERAL RESEARCH (J BONE MINER RES)
N6-methyladenosine methyltransferase plays a role in hypoxic preconditioning partially through the interaction with lncRNA H19-ACTA BIOCHIMICA ET BIOPHYSICA SINICA (ACTA BIOCH BIOPH SIN)
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